FA-12547 / Genomic coordinate mapping / Member archive
Reverse alignments apply soft clipping at the wrong query end · case 02
Original read positions map to the wrong reference base after reverse orientation and asymmetric soft clipping.
Case contract
For a synthetic ungapped alignment with leading/trailing soft clip lengths in alignment orientation, map a zero-based original read index to reference base or None. Query length is lead+aligned+trail. Reverse alignments reverse indexes across the entire query before clipping.
Why this case matters
An abstract offline coordinate model isolates this software mapping defect; it does not model biological interpretation.
One recorded failure
Sample boundary fixtureThis sample comes from the broken implementation of a controlled reproducer.
| Boundary fixture | Actual | Expected | Outcome |
|---|---|---|---|
| reverse first aligned original base | 102 | 103 | Failed |
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